Impedance spectroscopy for characterization of biological matter
Juan Jose Montero Rodriguez
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Juan Jose Montero Rodriguez, Impedance spectroscopy for characterization of biological matter (2018), Logos Verlag, Berlin, ISBN: 9783832590611
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Beschreibung / Abstract
Standard characterization methods of biological cells are time consuming and may reduce cell viability by staining them with markers. An alternative fast and non-destructive method is developed using impedance spectroscopy, which has potential applications in biology. The technique is used to identify tumor cells in mice, detect bacterial eye infections, monitor fruit ripening, and measure sweat lactate concentration in humans by using a skin sensor. These applications often require a portable measurement system. Therefore, three portable systems were designed and tested. It has been shown that the method can be further improved by four-terminal measurements. For extension of the method in the millimeter-wave frequencies, full electromagnetic simulation of the chip has been carried out, and electrodes and interconnections have been adjusted accordingly.
Beschreibung
Juan José Montero Rodríguez (born April 7, 1988 in San José, Costa Rica) studied electronic engineering and obtained the Licentiate degree in 2011 at the Instituto Tecnológico de Costa Rica, where he continued his formation in Microelectromechanical Systems (MEMS), and obtained the Master of Science degree in 2013. Then he started his doctoral degree studies in 2014 at the Institute of Nano- and Medical Electronics of the Hamburg University of Technology.
Inhaltsverzeichnis
- BEGINN
- 1 Introduction
- 1.1 Structure and classification of cells
- 1.2 History of animal electricity
- 1.3 Field exposure of cells and biological samples
- 1.4 Purpose of the work
- 1.5 Outline
- 2 Impedance spectroscopy
- 2.1 Impedance, permittivity and conductivity
- 2.2 Cellular impedance recording
- 2.3 Electrode configurations for EIS
- 2.4 Equivalent circuit components
- 2.5 Equivalent circuit models
- 2.6 Simulation of a 3D cell culture
- 2.7 Commercial tools for fitting
- 2.8 Impedance fitting toolbox
- 2.9 Chapter conclusion
- 3 Applications of impedance spectroscopy
- 3.1 Impedance response of tumor and healthy cells
- 3.2 Liquid samples infected with bacteria
- 3.3 Fruit ripening
- 3.4 Cell viability
- 3.5 Lactate concentration in athletes
- 3.6 Chapter conclusion
- 4 Impedance measurement circuits
- 4.1 Wheatstone bridge
- 4.2 Auto-balancing bridge method
- 4.3 Frequency response analysis method
- 4.4 Network analysis method
- 4.5 Chapter conclusion
- 5 Demonstrator of a portable impedance spectrometer
- 5.1 Block diagram
- 5.2 Design of the analog circuitry
- 5.3 Design of the digital logic
- 5.4 Frequency response measurements
- 5.5 Experiments with constant known loads
- 5.6 Experiments with yeast
- 5.7 Chapter conclusion
- 6 Miniaturized portable impedance measurement system
- 6.1 Chip architecture
- 6.2 Impedance calculation example
- 6.3 LabVIEW Implementation
- 6.4 Impedance measurements
- 6.5 Phase measurements
- 6.6 Biological experiments
- 6.7 Chapter conclusion
- 7 High frequency stimulation
- 7.1 Chip architecture
- 7.2 Impedance calculation
- 7.3 Simulations of an RC impedance load
- 7.4 DC operating point of the VCO
- 7.5 Inductor S-parameters
- 7.6 Oscillator measurements
- 7.7 Chapter conclusion
- 8 High frequency stimulation redesign
- 8.1 Chip architecture and simulations
- 8.2 Assembly technologies for bare die ASICs
- 8.3 Test board assembly
- 8.4 Oscillator frequency and amplitude
- 8.5 DC operating point of the VCO
- 8.6 Simulation of an ESD error
- 8.7 Measurement of cell cultures
- 8.8 Simulation of the microstrip line
- 8.9 Chapter conclusion
- 9 Conclusions and outlook
- A ASIC bonding plans
- Bibliography
- List of Figures
- List of Tables
- List of Abbreviations
- List of Symbols